University of North Florida
Browse the Citations
-OR-

Contact Info

Stuart Chalk, Ph.D.
Department of Chemistry
University of North Florida
Phone: 1-904-620-1938
Fax: 1-904-620-3535
Email: schalk@unf.edu
Website: @unf

View Stuart Chalk's profile on LinkedIn

Histidine

  • IUPAC Name: (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid
  • Molecular Formula: C6H9N3O2
  • CAS Registry Number: 71-00-1
  • InChI: InChI=1S/C6H9N3O2/c7-5(6(10)11)1-4-2-8-3-9-4/h2-3,5H,1,7H2,(H,8,9)(H,10,11)/t5-/m0/s1
  • InChI Key: HNDVDQJCIGZPNO-YFKPBYRVSA-N

@ ChemSpider@ NIST@ PubChem

Citations 5

"Turbulent Hydrodynamic Voltammetry. 3. Analytical Investigations With A Turbulent Voltammetric Cell And Applications To Amino Acid Analysis"
Anal. Chim. Acta 1977 Volume 94, Issue 2 Pages 351-356
M. Varadi and E. Pungor

Abstract: The analytical applicability of a turbulent voltammetric cell in a flowing stream has been studied. A silicone rubber-based graphite electrode is used. Under optimal conditions, the distortion of the signal in the cell as well as the fluctuations of the signal cause a relative standard deviation of 1% in the results. An examination of the amino acids which occur in protein hydrolyzates is reported. Proline, histidine and arginine can be determined with standard deviations of about 4%; the standard deviations range from 10% to 16% for the other amino acids, except threonine, serine and tyrosine which cannot be determined.
Electrode Voltammetry Turbulent flow

"Flow Injection Spectrophotometric Determination Of Amino-acids Based On An Immobilized Copper(II)-zincon System"
Anal. Chim. Acta 1993 Volume 281, Issue 3 Pages 601-605
L. Lahuerta Zamora, J. Martínez Calatayud

Abstract: The flow injection spectrophotometric determination of different amino acids was carried out by reaction with copper(II) ions entrapped in a polymeric material and filling a packed-bed reactor; the released copper(II), complexed with the amino acid, reacted with zincon in a basic medium producing a blue color that was monitored at 600 nm. The method was applied to determine the contents of different amino acids in pharmaceutical formulations. The calibration graph for glycine was a linear over the range 0.5-20 µg mL-11 with a relative standard deviation of 0.8% (n = 6) at 10 µg mL-1 and a sample throughput of 108 h1-1. The test solution (359 µL) was injected into a stream (2.7 ml/min) of 12.5 mM sodium tetraborate/4.6 mM HCl buffer of pH 9, which was passed through a reactor (13.5 cm x 1.5 mm i.d.; 150-200 µm) containing CuCO3.Cu(OH)2.2H2O and Co (as catalyst) entrapped in AL-100-A polyester resin (Reposa) prepared as described previously (cf. Ibid., 1992, 265, 81). The solution was then mixed with a stream (3.4 ml/min) of 0.1 mM zincon in the same buffer in a 30 cm reaction coil, and the absorbance of the released Cu(II)-zincon complex was measured at 600 nm. The calibration graph was linear from 0.5-20 µg/ml of glycine and the RSD was 0.85% (at 10 mg/ml); hourly throughput was 108 samples. Of the compounds that may be present in pharmaceuticals containing amino-acids, only ascorbic acid interfered (at 10 mg/ml of amino-acid and up to 500 mg/ml of interferent). The method was used successfully to determine glycine in phial contents and tablets, with RSD of 1 and 2.9%, respectively. The method was also applied in the determination of proline, histidine, alanine, leucine and phenylalanine (calibration data given).
Pharmaceutical Pharmaceutical Spectrophotometry Interferences Catalysis Solid phase reagent Resin Immobilized reagent

"Fluorimetric Determination Of Amino-acids And Proteins Utilizing A Copper(II)-catalysed Reaction"
Bull. Chem. Soc. Jpn. 1991 Volume 64, Issue 12 Pages 3634-3638
Hisakazu Mori,Kazue Sakai,Kyoko Yamashina,Sayuri Hirata and Kumiko Horie

Abstract: Amino-acids were found to accelerate the Cu(II)-catalyzed oxidation of di-2-pyridyl ketone hydrazone (I) to form a fluorescent compound in acidic medium. With use of this enhancement effect, L-histidine, L-cysteine, L-glutamic acid, glycine, DL-serine and L-arginine were determined by flow injection analysis (diagram given). Sample was injected into a carrier stream of water which was merged with stream of 0.5 µM I - 0.1 M NaOH, passed through a reaction coil operated at 25°C, and mixed with a stream of 0.4 M HCl before fluorimetric detection at 435 nm (excitation at 349 nm). The detection limit of L-histidine was 2 pmol. The method was also used to determine proteins which were found to decrease the catalysis; the detection limit for bovine serum albumin was 20 ng.
Fluorescence Catalysis

"Sensitive Determination Of Proteins By FIA With Coulometric Detection"
Bunseki Kagaku 1989 Volume 38, Issue 9 Pages 454-457
Kubo, H.;Huang, Y.S.;Kinoshita, T.;Nakazawa, H.

Abstract: Sample solution (5 ml) was injected into carrier/reagent solution comprising 0.3 M Na2HPO4 - 1 mM CuSO4 - 20 mM NH3 (pH 12) and passed to a reaction coil (5 m x 0.5 mm) operated at 95°C, and then to a cooling coil (1 m x 0.5 mm) operated at 10°C. Coulometric detection was carried out at 0.7 V. Calibration graphs were rectilinear for 10 to 100 ng of bovine serum albumin (I), human serum albumin, human γ-globulins and ovalbumin. The coefficient of variation (n = 10) at 10 and 25 ng of I were 3.5 and 3.1%, respectively. The detection limit for I was 2.5 ng. Histidine, cysteine, tryptophan and tyrosine were sensitively detected.
Cow Serum Serum Human Coulometry Heated reaction

"Determination Of An-fu-ming 11S Injection By HPLC - Post-column Derivatization"
Yaowu Fenxi Zazhi 1988 Volume 8, Issue 5 Pages 308-310
Zhong, P.

Abstract: A 1 mL injection sample (containing Thr, Gly, Val, Met, Ile, Leu, Phe, Lys, His and Arg) is diluted to 100 mL with 0.2N-Na citrate solution (pH 2.2) and then 1 mL of the solution is mixed with 1 mL of glutamic acid (100 µg mL-1; internal standard) and 0.2N-Na citrate to 10 mL. A 25 µL portion of solution is analyzed by HPLC on an ISC-07/S1540 strongly acidic ion-exchange column (15 cm x 4 mm) operated at 55°C with gradient elution (0.5 mL min-1) from 0.2N-Na citrate (pH 3.25) to 0.6N-Na citrate (pH 9). The eluate is passed (0.6 mL min-1) through a pre-heated (55°C) coil (60 cm x 0.3 mm) in which the amino-acids react with phthalaldehyde for 2.5 s. The derivatives are detected fluorimetrically. Recoveries are 98.2 to 102.3% and the coefficient of variation (n = 4) are 0.3 to 1.9%. No pre-treatment is required. The method is simple and accurate, with good reproducibility.
HPLC Fluorescence Heated reaction Post-column derivatization